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T7009

Sigma-Aldrich

Thrombin from human plasma

lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)

Synonym(s):

Factor IIa

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100 UNITS
€133.00
250 UNITS
€275.00
1000 UNITS
€667.00
5000 UNITS
€2,740.00

€133.00

In StockDetails

Diagnostics Manufacturers should use alternative grade product: SRE0003
Request a Bulk Order
All Photos(1)

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100 UNITS
€133.00
250 UNITS
€275.00
1000 UNITS
€667.00
5000 UNITS
€2,740.00

About This Item

CAS Number:
9002-04-4
Enzyme Commission number:
3.4.21.5 (BRENDA, IUBMB)
EC Number:
232-648-7
MDL number:
MFCD00082072
UNSPSC Code:
12352204
NACRES:
NA.54

€133.00

In StockDetails

Diagnostics Manufacturers should use alternative grade product: SRE0003
Request a Bulk Order

form

lyophilized powder

Quality Level

200

specific activity

≥1,000 NIH units/mg protein (E1%/280, 18.3)

mol wt

37.4 kDa

impurities

HIV and HBsAg, source material tested negative

UniProt accession no.

P00734

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

human ... F2(2147)

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General description

The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2.[1]

Application

Thrombin from human plasma has been:
  • dissolved in CaCl2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production[2]
  • to polymerize fibrin on well plates[3]
  • used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells[4]

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Thrombin is an endolytic serine protease[5] which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor.[6] It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B.[5] The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer.[5] Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500.[7]

Unit Definition

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

inhibitor

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000410046
0000389789
0000371958
0000371961
0000350764

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Epidemiology and pathophysiology of cancer-associated thrombosis
Noble S and Pasi J
British Journal of Cancer, 102(S1), S2-S2 (2010)
Integrin alphavbetabgr3-RGDS interaction mediates fibrin-induced morphological changes of glomerular endothelial cells
Xu Q, et al.
Kidney International, 56(4), 1413-1422 (1999)
Signal transduction for proteinase-activated receptor-2-triggered prostaglandin E2 formation in human lung epithelial cells
Kawao N, et al.
Journal of Pharmacology and Experimental Therapeutics, 315(2), 576-589 (2005)
B J Biemond et al.
Circulation, 96(5), 1612-1615 (1997-10-07)
In many case-control as well as epidemiological studies, increased lipoprotein(a) [Lp(a)] levels are considered to constitute an independent risk factor for premature coronary artery and cerebrovascular disease. Lp(a) resembles an LDL particle with an additional linked protein [apolipoprotein(a), apo(a)], whose
Capillary-like network formation by human amniotic fluid-derived stem cells within fibrin/poly (ethylene glycol) hydrogels
Benavides OM, et al.
Tissue Engineering: Part A, 21(7-8), 1185-1194 (2015)
View All Related Papers

Articles

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

Questions

1–8 of 8 Questions  

  1. How much is 100UN of thrombin(T6884)? what is the weight in mg?

    1 answer

    1. Both of these Thrombin products - T7009 and T6884 - are sold by activity or units. The amount of powder in the vial is lot specific and based on the activity reported in the Certificate of Analysis.

      T6884-100UN, lot SLCP0517 tested at 2736 uits per milligram. The 100UN vial contains 36ug of powder per vial. See the link below to review this Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T6884-100UNSLCP0517.pdf

      T7009-100UN, lot SLCN9908 tested at 1742 units per milligram. The 100UN vial contains 57ug of powder per vial. See the link below to review this Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T7009-250UNSLCN9908.pdf

      Helpful?

  2. How much does the T-7009-100UN weigh with and without packaging? Country of origin Germany?

    1 answer

    1. This product is packaged in units, the exact amount of product will depend on the lot-specific activity. For example, the SLCN9908 has a specific activity of 1742 units/ mg protein. The end user will receive 0.06 mg. The net weight of packaging is 1,000 mg. The product is manufactured in the USA.

      Helpful?

  3. What is the isoelectric point of Product T7009, Thrombin from human plasma?

    1 answer

    1. The isoelectric point (pI) range of the human thrombin isozymes is 6.35 - 7.6. The major isoform has a pI of 7.31.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. For thrombin, how do NIH units compare to International Units (IU)?

    1 answer

    1. One NIH unit of thrombin is equivalent to approximately 1.8 International Unit (IU) of thrombin.

      Helpful?

  6. What is the solution stability of Product T7009, Thrombin from human plasma?

    1 answer

    1. By analogy to thrombin from bovine plasma (T4648), we expect stock solutions of thrombin from human plasma to be stable for one week kept refrigerated at 2-8° C. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store frozen at -20°C or colder for long term storage.

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  7. What is the solubility of Product T7009, Thrombin from human plasma?

    1 answer

    1. Thrombin from human plasma gave a clear very faint yellow solution when dissolved at 100 unit vial plus 1 ml of 28 mM sodium barbital, 0.125M sodium chloride, 1% BSA, 0.5% PEG, pH 7.35.  Thrombin from bovine plasma (T4648) is soluble in water at 10 mg/mL.

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  8. What is the cross reactivity of Thrombin?

    1 answer

    1. Thrombin from any vertebrate species will clot the fibrinogen of virtually any other species.  Fibrinogen from any mammalian source will cross-react with thrombin from any mammalian source, but it is not well known how thrombin and fibrinogen from different animals differ in their sequence.  When any mammalian thrombin is injected into a different mammal, clotting will occur.

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