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STRN250

Sigma-Aldrich

Spectrum™ Plant Total RNA Kit

greener alternative

sufficient for 250 purifications

Synonym(s):

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

usage

sufficient for 250 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

RNA purification: suitable

greener alternative category

Aligned,

storage temp.

15-25°C

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General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Spectrum®Plant Total RNA Kit has been used:
  • to extract RNA from the material ground in liquid nitrogen
  • to isolate total RNA from mutant and wt (wild type) plantlets
  • to extract total RNA from leaf samples
  • to extract RNA from all plant tissues

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

Specac is a registered trademark of Specac Ltd.
Spectrum is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Target Organs

Liver,Heart

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Certificates of Analysis (COA)

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Gregory S Jensen et al.
Journal of experimental botany, 68(3), 499-511 (2017-02-17)
Thigmomorphogenesis is a stereotypical developmental alteration in the plant body plan that can be induced by repeatedly touching plant organs. To unravel how plants sense and record multiple touch stimuli we performed a novel forward genetic screen based on the
Zhonghua Li et al.
The New phytologist, 226(6), 1738-1752 (2020-02-06)
The cotton fibre serves as a valuable experimental system to study cell wall synthesis in plants, but our understanding of the genetic regulation of this process during fibre development remains limited. We performed a genome-wide association study (GWAS) and identified
Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness
Minina EA, et al.
Journal of Experimental Botany, 69(6), 1415-1432 (2018)
Anantha Peramuna et al.
Biochemical and biophysical research communications, 500(2), 418-422 (2018-04-17)
Securing a molecular toolbox including diverse promoters is essential for genome engineering. However, native promoters have limitations such as the available number or the length of the promoter. In this work, three short synthetic promoters were characterized by using the
Martin-Timothy O'Donoghue et al.
Journal of experimental botany, 64(12), 3567-3581 (2013-07-28)
Bryophytes, the most basal of the extant land plants, diverged at least 450 million years ago. A major feature of these plants is the biphasic alternation of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. These
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Related Content

Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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