SCP0252

Thrombin Substrate, fluorogenic

• Empirical Formula (Hill Notation): C₃₁H₄₂N₇O₇
• Molecular Weight: 624.71
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• Assay: ≥95% (HPLC)
• form: lyophilized
• composition: Peptide Content, ≥80%
• storage condition: protect from light
• storage temp.: −20°C

Description

Amino Acid Sequence

BOC-Val-Pro-Arg-AFC

General description

Thrombin has procoagulant and anticoagulant properties. It also has chemotactic and mitogenic effects. Thrombin triggers the growth of mammalian cells, fibroblasts and macrophage- like tumor cell lines. It regulates brain development. Thrombin plays an important role in hemostasis and thrombosis. It is associated with mast cell degranulation along with the release of histamine and enhances the production of IL-1 (interleukin-1) by activated macrophages. Thrombin stimulates vascular permeability and transendothelial cell migration by neutrophils.

Application

Thrombin is a serine protease involved in the coagulation cascade wherein it converts fibrinogen into fibrin. Thrombin may be detected (assayed) using peptides that contain the VPR sequence such as the fluorogenic substrates Boc-VPR-AFC, Boc-Val-Pro-Arg-7-AMC and the chromogenic substrate Boc-Val-Pro-Arg-pNA. These substrates may also be useful to detect other serine proteases.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable