SAB4200753

Anti-phospho-AKT (pThr⁴⁵⁰) antibody, Mouse monoclonal

clone AK-11, purified from hybridoma cell culture

• Synonym(s): Anti-Protein Kinase B, Anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AK-11, monoclonal
• form: buffered aqueous solution
• mol wt: ~56 kDa
• species reactivity: mouse, chicken, rat, human
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract; immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells; immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
• isotype: IgG2a
• UniProt accession no.: P31749
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pThr450)
• Gene Information: human ... AKT1(207)

Description

General description

Anti-phospho-AKT (pThr450) antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the AK-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Serine/threonine kinase (AKT) also known as protein kinase B (PKB) belongs to the family of serine/threonine kinases. It consists of three isoforms such as AKT1, AKT2 and AKT3. AKT1 gene is located on the human chromosome at 14q32.33.

Specificity

Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal specifically recognizes all three AKT isoforms phosphorylated at Thr450 (AKT1, AKT2 and AKT3).

Immunogen

Synthetic peptide corresponding to human AKT1 pThr450

Application

Anti-phospho-AKT (pThr450) antibody, mouse monoclonal may be used in:

• immunoblotting
• immunofluorescence
• immunoprecipitation

Biochem/physiol Actions

Serine/threonine kinase (AKT) plays an important role in the control of cell cycle and cell proliferation. It is also involved in cell differentiation and apoptosis signaling pathways. AKT is rapidly activated in response to cell stimulation by several growth factors, such as insulin, peroxyvanadate or cellular stresses (such as heat shock). Following activation, the phosphorylated AKT is relocated to the nucleus. Lack of phosphorylation at Thr450 enhances AKT ubiquitination and degradation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable