SAB4200662

Monoclonal Anti-SPTBN1 antibody produced in mouse

clone FOD5, hybridoma cell culture supernatant

• Synonym(s): EIEE5, NEAS, SPTA2, alpha, non-erythrocytic 1, spectrin
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: FOD5, monoclonal
• form: buffered aqueous solution
• species reactivity: human, bovine, rat, canine
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 1:100-1:200 using HeLa cells; immunoblotting: 1:500-1:1000 using whole extracts of SH-SY5Y cells; immunofluorescence: 1:500-1:1000 using using methanol:aceton fixed MDA-MB-231 cells.; immunohistochemistry: suitable
• UniProt accession no.: Q01082
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SPTBN1(6711) ; rat ... Sptbn1(305614)

Description

General description

Monoclonal Anti-SPTBN1 (mouse IgM isotype) is derived from the hybridoma FOD5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human SPTBN1, conjugated to KLH. SPTBN1 (Spectrin-beta, non-erythrocytic 1 chain) also known as β-Fodrin, is an important cytoskeletal protein. Spectrin is a heterodimer, consist of two subunits, α (SPTAN1) and β (SPTBN1). The prototypical SPTBN1 contains an N-terminal actin-binding domain, 17 triple helix Spectrin repeats, and a C-terminal pleckstrin homology (PH) domain.

Immunogen

a synthetic peptide corresponding to an internal region of human SPTBN1

Application

Monoclonal Anti-SPTBN1 antibody has been used in

• immunoblotting
• immunofluorescence
• flow cytometry
• immunohistochemistry

Biochem/physiol Actions

Spectrin forms a complex network by interacting with intracellular proteins such as actin, ankyrin, and adducin. Spectrin has an important role in maintaining the stability, structure, shape of the cell. It also maintains the arrangement of transmembrane proteins, and organization of organelles. Furthermore, Spectrin is involved in diverse cell functions such as cell adhesion, cell spreading, and the cell cycle. Ankyrin interacts with SPTBN1 repeats 14 and 15. Mutations in SPTBN1 have been linked to hereditary elliptocytosis, a type of haemolytic anaemia, spinocerebellar ataxia type 5 and cancer.

Physical form

Supplied as a culture supernatant solution containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable