SAB4200379

Anti-JMY antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Junction mediating and regulatory protein, Anti-p53 cofactor
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~120 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of human WiDr cells.; indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.; western blot: 0.5-1.0 μg/mL using whole extracts of human WiDr cells
• UniProt accession no.: Q8N9B5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... JMY(133746)

Description

General description

Junction mediating and regulatory protein (JMY) is identified as a transcriptional co-activator of p53 and is a multifunctional actin nucleation factor. JMY contains Wiskott-Aldrich syndrome protein (WASp)-homology 2 (WH2) domains in its C-terminus. The JMY gene is mapped to human chromosome location 5q14.1.

Specificity

Anti-JMY recognizes human JMY.

Immunogen

synthetic peptide corresponding to the C-terminal region of human JMY, conjugated to KLH.

Application

Anti-JMY antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence.

Biochem/physiol Actions

Junction mediating and regulatory protein (JMY) acts as a nucleation promoting factor by activating actin-related proteins-2/3 (ARP2/3), creating branched filament networks, and also by assembling unbranched filaments directly through a Spire-like mechanism. Overexpression of JMY increases motility while its loss slows cell migration. DNA damage causes JMY to accumulate in the nucleus, where it enhances p53-dependent transcription of pro-apoptotic genes. The activities of JMY as a p53 co-factor or as an actin nucleation factor are regulated by its subcellular localization.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable