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S5134

Sigma-Aldrich

Scott′s Tap Water Substitute Concentrate

10 ×

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6 X 100 ML
€203.00

€203.00

Available to ship on11 April 2025Details

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6 X 100 ML
€203.00

About This Item

UNSPSC Code:
41116124
NACRES:
NA.47

€203.00

Available to ship on11 April 2025Details

Request a Bulk Order

form

powder

IVD

for in vitro diagnostic use

concentration

10 ×

technique(s)

titration: suitable

pH

7.0-7.8

application(s)

hematology
histology

storage temp.

room temp

Related Categories

Application

Used as a "blueing reagent" in hematoxylin and eosin staining procedures.[1] Scott′s Tap Water Substitute Concentrate has been used in transferase−mediated dUTP nick end labeling (TUNEL) assay.[2] It has been used in immunohistochemical analysis.[3]

Other Notes

MgSO4 • 7H2O, 200 g/L + NaHCO3, 20 g/L

Preparation Note

Scott′s Tap Water Substitute is prepared by mixing 1 part of Scott′s Tap Water Substitute Concentrate with 9 parts deionized water.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM0502
SLCP6932
SLCF8585
SLBW6049
SLCB7313

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Questions

1–3 of 3 Questions  

  1. Product 65354-85 has been deleted. What is a suitable replacement product?

    1 answer

    1. Suitable replacements for the deleted Blueing Reagent (65354-85) are S5134 and 106329. Scott's Tap Water Concentrate is diluted 1 to 10 before use. 106329 is typically dissolved in water at a concentration of 15 grams per liter. It is also possible to use a 0.2% solution of ammonium hydroxide or a saturated solution of lithium carbonate.

      Helpful?

  2. Is there a direct replacement for S5134 Scott's Tap Water Substitute?

    1 answer

    1. There is no direct replacement for the product in question. However, a solution with 15 grams of sodium bicarbonate per liter of water serves exactly the

      Helpful?

  3. Must Scott's Tap Water Substitute be diluted with deionized water, and must the solution be prepared fresh for each day?

    1 answer

    1. The Scott's Tap Water Substitute Concentrate should be diluted with deionized water. When using distilled water, it is advisable to check the pH after preparing the 1X solution. Alternatively, the solution prepared with distilled water can be used to blue tissue sections after staining with hematoxylin. If the nuclei do not turn blue, it may indicate that the pH of the solution is not alkaline enough to convert the nuclei color to blue.

      Helpful?

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