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RAB0327

Sigma-Aldrich

Human Insulin ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

Insulin

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 μIU/mL
standard curve range: 4.69-300 μIU/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... INS(3630)

General description

The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Insulin

Application

For research use only. Not for use in diagnostic procedures.

Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum and HAMECs (human adipose microvascular endothelial cells) which were isolated from human visceral fat of nondiabetic patients.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Insulin ELISA Kit has been used:
  • to measure the blood levels of human insulin and proinsulin in the transgenic mice
  • to measure serum insulin indaibetic rats
  • to measure insulin in Multiple Sclerosis patients

Biochem/physiol Actions

Insulin is responsible for two types of actions, excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis. Insulin plays a vital role in regulation of blood glucose level within the normal range. Abnormal insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycemia. Type I diabetes is a result of autoimmune destruction of β cells of pancreas, which leads to depletion of insulin. Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding. Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Production of recombinant human proinsulin in the milk of transgenic mice.
Qian X
Scientific Reports, 4:6465 (2014)
Xi Qian et al.
Scientific reports, 4, 6465-6465 (2014-10-01)
There is a steady increasing demand for insulin worldwide. Current insulin manufacturing capacities can barely meet this increasing demand. The purpose of this study was to test the feasibility of producing human proinsulin in the milk of transgenic animals. Four
Bo Liu et al.
Molecular medicine reports, 24(1) (2021-05-14)
The present study sought to elucidate the role of Polygonatum sibiricum F. Delaroche polysaccharide (PSP) in high‑fat diet (HFD)‑induced mouse obesity and investigated the primary molecular mechanism underlaying these effects. An obese mouse model was established by feeding HFD and
Ginger extract attenuates preliminary steps of streptozotocin-mediated oxidative stress in diabetic rats.
Waly M I, et al.
International journal of nutrition, pharmacology, neurological diseases., 5(4), 151-151 (2015)
Production of recombinant human proinsulin in the milk of transgenic mice
Qian X, et al..
Scientific Reports, 4, 6465-6465 (2014)
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