R8903

Monoclonal Anti-hnRNP-K/J antibody produced in mouse

clone 3C2, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Heterogeneous Nuclear Ribonucleoprotein K/J
• MDL number: MFCD06798704
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3C2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: hamster, Xenopus, bovine, human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.1-0.2 μg/mL using total cell extract of MBDK cells
• isotype: IgG2b
• UniProt accession no.: P61978
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HNRNPK(3190)

Description

General description

Monoclonal Anti-hnRNP-K/J (mouse IgG2b isotype) is derived from the 3C2 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with hnRNP′s purified by oligo (dC) affinity chromatography.

Immunogen

hnRNPs purified by oligo (dC) affinity chromatography.

Application

The antibody may be used in various immunochemical techniques including enzyme linked immunosorbent assay (ELISA), immunoblotting, chromatin immunoprecipitation (ChIP), immunocytochemistry, antibody-based supershift assay and electrophoretic mobility shift assay (EMSA).

Biochem/physiol Actions

Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-K is found both in the nucleus, cytoplasm, and mitochondria, therefore, it was found to be involved in many biological events in the cell like: transcription, chromatin remodeling, splicing and translation. hnRNP-K is regulated by different signaling cascades and can bind kinases. As a consequence, it can mediate signals from the cytoplasm to the nucleus.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable