I bought this kit as replacement of NA0410-1Kt which I was waiting for 1 year and still wasn't able to get due to delay. Plex15 is significantly more expensive, uncomfortable to work with and yields less plasmid DNA. I regret I had to waste my time and money on it.
PLEX15
GenElute™ Endotoxin-free Plasmid Maxiprep Kit
GenElute™ Endotoxin-free Plasmid Maxiprep Kit
sufficient for 15 purifications
Synonym(s):
GenElute™ Endotoxin-free Plasmid Kits, Gen Elute
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1 KIT
€558.00
€558.00
Estimated to ship on29 March 2025
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1 KIT
€558.00
About This Item
UNSPSC Code:
41105501
NACRES:
NA.52
€558.00
Estimated to ship on29 March 2025
Recommended Products
usage
sufficient for 15 purifications
Quality Level
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
greener alternative category
, Aligned
storage temp.
15-25°C
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 μg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 μg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
Application
Features and Benefits
- High transfection efficiency and yield
- Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
- Fast and simple allowing up to 12 preps in less than 2 hours
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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