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MAK057

Sigma-Aldrich

Citrate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Citrate Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Citrate is a key Tricarboxylic Acid (TCA) cycle intermediate formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA. Citrate is transported out of the mitochondria via the citrate-malate shuttle and converted back to acetyl-CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis via its actions on acetyl-CoA carboxylase and of glycolysis via its actions on phospho- fructokinase. Citrate metabolism and disposition can vary widely due to sex, age, and a variety of other factors including disease states. Cellular citrate levels are decreased in prostrate cancer cells and citrate levels may be a marker of prostrate cancer growth rate. The Citrate Assay Kit provides a simple, sensitive, and rapid means of quantifying citrate in a variety of samples.

Application

Citrate Assay kit has been used to determine the concentration of citrate in samples.[1][2][3]

Suitability

Suitable for the measurement of citrate in a variety of samples including tissue and cells

Principle

Citrate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the citrate present. Typical sensitivites of the Citrate Assay Kit are between 0.1 to 10 nmoles (2 μM-10 mM) of citrate in a variety of samples.

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Description
Pricing

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
8M13K06550
8J28K06550
8B08K06550
7F22K06550
6L09K06550

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Di Zhu et al.
Science advances, 6(31), eabb2529-eabb2529 (2020-08-14)
Mild mitochondrial stress experienced early in life can have beneficial effects on the life span of organisms through epigenetic regulations. Here, we report that acetyl-coenzyme A (CoA) represents a critical mitochondrial signal to regulate aging through the chromatin remodeling and
Ricardo Villa-Bellosta et al.
Scientific reports, 9(1), 11374-11374 (2019-08-08)
Vascular calcification is highly prevalent in patients with chronic hemodialysis. Increased acetatemia during hemodialysis sessions using acetate-acidified bicarbonate has also been associated with several abnormalities, By contrast, these abnormalities were not induced by citrate-acidified bicarbonate dialysis. Moreover, citrate is biocompatible
Jade D Bailey et al.
Cell reports, 28(1), 218-230 (2019-07-04)
Classical activation of macrophages (M(LPS+IFNγ)) elicits the expression of inducible nitric oxide synthase (iNOS), generating large amounts of NO and inhibiting mitochondrial respiration. Upregulation of glycolysis and a disrupted tricarboxylic acid (TCA) cycle underpin this switch to a pro-inflammatory phenotype.
Nimrit Goraya et al.
Kidney international, 95(5), 1190-1196 (2019-03-09)
Acid (H+) retention appears to contribute to progressive decline in glomerular filtration rate (GFR) in patients with chronic kidney disease (CKD), including some patients without metabolic acidosis. Identification of patients with H+ retention but without metabolic acidosis could facilitate targeted
Yan Huang et al.
Regenerative biomaterials, 7(2), 221-232 (2020-04-17)
The purpose of this article was to explore the effects of gold nanoparticles (GNPs) and silver nanoparticles (SNPs) with different cytotoxicities on human dermal fibroblasts (HDFs) at the metabolic level. First, ∼20 nm of GNPs and SNPs were prepared, and their
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Articles

Fatty Acid Synthesis in Cancer Cells

Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.

Questions

  1. How are the detection range and sensitivity determined for the MAK057 assay?

    1 answer

    1. The calculation of the higher limit of detection is determined by dividing the highest concentration of the standard by the minimum sample volume. Similarly, the lower limit of detection is calculated by dividing the lowest concentration standard by the maximum sample volume. In the event that a sample exceeds the range of the standard curve, it is advisable to conduct a dilution and then adjust for the dilution factor.
      For instance, for the MAK057 test:

      Colorimetric:
      • Lower range: 2 nmol/50 = 0.04 mM or 40 uM
      • Higher range: 10 nmol/1 uL = 10 mM or 10,000 uM
      Fluorometric:
      • Lower range: 0.2 nmol/50 = 0.004 mM or 4 uM
      • Higher range: 1 nmole/1 uL = 1 mM or 1000 uM

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