M5566

Anti-Myosin IX/Myr5 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CELIAC4, Anti-MYR5
• MDL number: MFCD04974766
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 230 kDa
• species reactivity: rat
• technique(s): microarray: suitable; western blot: 1:1,000 using rat embryonic brain extract.
• UniProt accession no.: Q63358
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Myo9b(25486)

Description

General description

Myosin IX belongs to the family of unconventional myosins. The tail region of these myosins contains a GTPase-activating protein (GAP) domain. It is expressed at higher levels in the embryonic brain than in the adult brain. In the adult rat, it is highly expressed in the lung, liver, spleen, and testis. The subcellular localization of class IX myosins appears to be partly cytoplasmic and partly associated with membranes and the actin cytoskeleton. The tail domain of myosin IX/Myr5 contains a region that coordinates two atoms of zinc. Myosin IX is thought to be involved in signal transduction and leukocyte differentiation.

Myosin IX/Myr5 is a single headed myosin which possesses an N-terminal extension and a large loop 2 insertion.

Specificity

Anti-Myosin IX/Myr5 recognizes rat myosin IX/Myr5 (230 kDa).

Immunogen

synthetic peptide corresponding to the C-terminal region of rat myosin IX/Myr5 (amino acids 1960-1980).

Application

Anti-Myosin IX/Myr5 antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Myosin IX expression level may vary during development and differentiation. The tail domain of myosin IX/Myr5 and the GTPase-activating protein (GAP) domain stimulates GTP hydrolysis. It is implicated in the regulation of actin organization, and the actin cytoskeleton. Overexpression of both Myr5 and Myr7 in cultured cells results in inactivation of Rho, loss of actin stress fibers, and focal contacts, leading to changes in cell morphology.

Myosin IX/Myr5 moves along the actin filaments for long distances and is mostly present in its ATP-bound state. It has been studied as a negative regulator of RhoC which is a G-protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.