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L5006

Sigma-Aldrich

Leucine Aminopeptidase, microsomal from porcine kidney

Type IV-S, ammonium sulfate suspension, 10-40 units/mg protein (Bradford)

Synonym(s):

Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase

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About This Item

CAS Number:
9054-63-1
Enzyme Commission number:
3.4.11.2 (BRENDA, IUBMB)
EC Number:
232-942-5
MDL number:
MFCD00131501
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type IV-S

form

ammonium sulfate suspension

specific activity

10-40 units/mg protein (Bradford)

storage temp.

2-8°C

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General description

Leucine Aminopeptidase belongs to the family of metalloprotease.

Application

Leucine Aminopeptidase, microsomal from porcine kidney has been used in the isolation of neprilysin (NEP) from rodent brain-C5 and for peptide digestion.

Biochem/physiol Actions

Leucine Aminopeptidase possesses broad substrate-specificity. It hydrolyzes peptides up to the proline residue and does not continue to degrade beyond proline.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

Physical form

Suspension in 3.5 M (NH4)2SO4 solution, pH 7.7, containing 10 mM MgCl2

inhibitor

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Intestinal digestive resistance of immunodominant gliadin peptides
Hausch F, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 283(4), G996-G1003 (2002)
The most potent organophosphorus inhibitors of leucine aminopeptidase. Structure-based design, chemistry, and activity
Grembecka J, et al.
Journal of medicinal chemistry, 46(13), 2641-2655 (2003)
Rossella Galati et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 58(7-8), 558-561 (2003-08-27)
The ability of synthetic protein fragments to survive the degradative action of aminopeptidases and serum proteolytic enzymes can be remarkably enhanced by slight modifications at their N-terminal alpha-amino group. This can be achieved by addition of beta-alanine or amino acids
L Willemijn Vader et al.
Gastroenterology, 125(4), 1105-1113 (2003-10-01)
Celiac disease is caused by T-cell responses to wheat gluten-derived peptides. The presence of such peptides in other widely consumed grains, however, has hardly been studied. We have performed homology searches to identify regions with sequence similarity to T-cell stimulatory
Johanna Rankenberg et al.
Experimental eye research, 210, 108704-108704 (2021-07-25)
Advanced glycation end products (AGEs) accumulate with age in human lens capsules. AGEs in lens capsules potentiate the transforming growth factor beta-2-mediated mesenchymal transition of lens epithelial cells, which suggests that they play a role in posterior capsule opacification after
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