L4025

Lyticase from Arthrobacter luteus

lyophilized powder, ≥200 units/mg solid

• Synonym(s): (1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme
• CAS Number: 37340-57-1
• MDL number: MFCD00131560
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Arthrobacter luteus)
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥200 units/mg solid
• technique(s): cell based assay: suitable
• suitability: suitable for cell lysis
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Description

Application

Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.

Lyticase from Arthrobacter luteus has been used:

• as a component in spheroplasting buffer to prepare yeast nuclear extracts[1]
• for digestion in sample preparation for β-glucan enzymatic assay using yeast[1]
• to lyse cells for RNA isolation[2]

Biochem/physiol Actions

Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation.[3] Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities.[4] It is useful with yeast cells such as e.g. Candida,[5] Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.

Unit Definition

One unit will produce a ΔA₈₀₀ of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves