KCQS02

KiCqStart^® SYBR^® Green qPCR ReadyMix^™

with ROX^™ for ABI instruments

• Synonym(s): qPCR master mix, sybr green qPCR
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• form: liquid
• usage: sufficient for 1250 reactions; sufficient for 250 reactions; sufficient for 5000 reactions
• feature: dNTPs included; hotstart
• storage condition: protect from light
• technique(s): qPCR: suitable
• color: colorless
• input: purified DNA
• compatibility: for use with ABI 5700; for use with ABI 7000; for use with ABI 7300; for use with ABI 7700; for use with ABI 7900 HT Fast; for use with ABI 7900 HT; for use with ABI 7900; for use with ABI StepOne; for use with ABI StepOnePlus
• detection method: SYBR^® Green
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Application

KiCqStart^® SYBRSYB^® Green qPCR ReadyMix^™ has been used:

• in the amplification and quantification of cDNA reverse transcribed from RNA extracted from mice brain samples in a 2-step RT-qPCR assay
• to analyze DNA purified by ChIP technique
• to perform gene expression analysis
• in amplification of RNA isolated from hearts of adult TL wild-type fish by quantitative real-time polymerase chain reaction (PCR)

PCR applications:

• Gene expression
• DNA quantification
• CHiP

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs, (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Other Notes

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Legal Information

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

KiCqStart is a registered trademark of QIAGEN Beverly Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable