indirect immunofluorescence: 1:100 using rat pheochromocytoma PC12 cell line differentiated with NGF microarray: suitable western blot: 1:1,000 using mouse brain cytosolic fraction
KIF17 belongs to the functionally diverse subgroup of the kinesin superfamily characterized by a N-terminal motor domain. KIF17 is specifically expressed in the brain, abundantly present in the gray matter, particularly in the hippocampus and cerebral cortex, but not in the white matter such as the optic nerve. KIF17, a dendritic specific motor protein, is localized in dendrites of pyramidal neurons, but not in axons or nuclei of the cerebral cortex.
Immunogen
synthetic peptide corresponding to amino acids 589-606 of mouse KIF17 conjugated to KLH.
Application
Anti-KIF17 antibody produced in rabbit has been used in immunofluorescence and immunoblotting.
Biochem/physiol Actions
The kinesin superfamily of proteins (KIFs) consists of a class of microtubule-dependent motors. They play a major role in many cellular and developmental functions. The kinesin proteins are involved in organelle transport and are primarily associated with anterograde transport of vesicles and organelles in neurons, epithelial cells, and melanosomes with bidirectional transport of mitochondria. They also mediate transport between the endoplasmic reticulum (ER) and the Golgi complex. In neurons, kinesin motors conduct vesicular transport, such as of synaptic vesicle components to axons and of neurotransmitter receptors to dendrites. KIF17 conveys (in dendrites) specific cargo vesicles that contain the glutamate receptor NR2B subunit, thus KIF17 functions as the sorting machinery for NR2B.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Current biology : CB, 26(13), 1705-1712 (2016-06-07)
Kinesin and dynein motors drive bidirectional cargo transport along microtubules and have a critical role in polarized cargo trafficking in neurons [1, 2]. The kinesin-2 family protein KIF17 is a dendrite-specific motor protein and has been shown to interact with
Interplay of PIWI/Argonaute protein MIWI and kinesin KIF17b in chromatoid bodies of male germ cells
Kotaja N, et al.
Journal of Cell Science, 119(13), 2819-2825 (2006)
KIF17 dynamics and regulation of NR2B trafficking in hippocampal neurons
Guillaud L, et al.
The Journal of Neuroscience, 23(1), 131-140 (2003)
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