G7126

Glycine

≥99% (HPLC), suitable for western blotting, ReagentPlus^®

• Synonym(s): Aminoacetic acid, Aminoethanoic acid, Glycocoll
• Linear Formula: NH₂CH₂COOH
• CAS Number: 56-40-6
• Molecular Weight: 75.07
• Beilstein: 635782
• EC Number: 200-272-2
• MDL number: MFCD00008131
• UNSPSC Code: 12352209
• eCl@ss: 32160406
• PubChem Substance ID: 24895290
• NACRES: NA.26

Properties

• Product Name: Glycine, ReagentPlus^®, ≥99% (HPLC)
• Quality Level: 200
• product line: ReagentPlus^®
• Assay: ≥99% (HPLC)
• form: powder
• technique(s): western blot: suitable
• impurities: ≤0.01% Ammonia
• loss: ≤0.2% loss on drying
• color: white to off-white
• pKa (25 °C): (1) 2.35, (2) 9.60; 2.35
• mp: 240 °C (dec.) (lit.)
• solubility: H₂O: 200 mg/mL, clear, colorless to faintly yellow
• cation traces: ≤0.01% (Ammonia (NH3))
• functional group: amine; carboxylic acid
• storage temp.: room temp
• SMILES string: NCC(O)=O
• InChI: 1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)
• InChI key: DHMQDGOQFOQNFH-UHFFFAOYSA-N
• Gene Information: rat ... Grin2a(24409)

Description

General description

Glycine is a non-chiral amino acid.[1] Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking region in polyacrylamide gels. It has advantage of low mobility, hydrophobicity and it does not associate with proteins.[2]

Glycine is a proteinogenic amino acid that plays a diverse array of roles in biochemistry and physiology. This non-essential, non-polar glucogenic amino acid acts as an inhibitory neurotransmitter in the central nervous system, but also works alongside glutamate as a co-agonist at NMDA receptors. As a precursor, glycine serves as a building block for various macromolecules, playing a crucial role in cellular processes.

Beyond its vital biological functions, glycine demonstrates remarkable versatility in various research applications. Its application as a biochemical reagent makes it invaluable in numerous assays and procedures. Its buffering capacity allows it to play a critical role in protein analysis techniques like SDS-PAGE and Western Blotting, chromatography, and cell culture. Glycine′s zwitterionic nature makes it an effective buffer across a range of pH values. In immunology research, it is widely used in the preparation of buffers for Western Blotting and other techniques. Additionally, its compatibility with various enzymes makes it useful in enzymatic assays like lactate determination. Furthermore, glycine contributes to the formulation of buffers for protein stabilization, pH control, and enzymatic reactions.

Application

• Glycine has been added in the transfer buffer during western blotting procedure.[3]
• It has been used in the solution prepared for dissolving formazan crystals in the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) cytotoxicity assay.[4]
• It has been used for terminating the rat basophilic leukemia (RBL) assay.[5]

Biochem/physiol Actions

Glycine is an inhibitory neurotransmitter in the central nervous system and works as an allosteric regulator of NMDA (N-methyl-D-aspartate) receptors. It is involved in processing of motor and sensory data, thereby regulating movement, vision and audition.[6]

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Features and Benefits

• Suitable for Cell Biology and Biochemical research
• High-quality compound suitable for multiple research applications

Other Notes

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Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves