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B9673

Sigma-Aldrich

1-Bromo-3-chloropropane

for isolation of RNA

Synonym(s):

1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide

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About This Item

Linear Formula:
Cl(CH2)3Br
CAS Number:
109-70-6
Molecular Weight:
157.44
Beilstein:
605278
EC Number:
203-697-1
MDL number:
MFCD00000998
UNSPSC Code:
41105515
PubChem Substance ID:
24892149
NACRES:
NA.52

grade

for molecular biology

Assay

≥98.5% (GC)

form

liquid

refractive index

n20/D 1.486 (lit.)

bp

144-145 °C (lit.)

density

1.592 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

ClCCCBr

InChI

1S/C3H6BrCl/c4-2-1-3-5/h1-3H2

InChI key

MFESCIUQSIBMSM-UHFFFAOYSA-N

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General description

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Application

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
  • feline teeth
  • mice brain and liver tissues
  • human ectocervical epithelial cells
  • placental samples
  • human mesenchymal stromal cells (hMSCs)

Features and Benefits

  • less toxic alternative to chloroform
  • compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Other Notes

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

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Description
Pricing

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Statements

H302,H331,H335,H341,H412

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isolation of total RNA from transgenic mouse melanoma subsets using fluorescence-activated cell sorting
Microarray methods for drug discovery, 27-44 (2010)
Neuroprotection Induced by Energy and Protein-Energy Undernutrition Is Phase-Dependent After Focal Cerebral Ischemia in Mice
de Carvalho TS, et al.
Translational Stroke Research, 1-12 (2019)
Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4
Lashkari BS and Anumba DOC
PLoS ONE, 12(3), e0173646-e0173646 (2017)
ALCAM (CD166) as a gene expression marker for human mesenchymal stromal cell characterisation
Brinkhof, et al.
Gene: X, 5, 100031-100031 (2020)
Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation.
P Chomczynski et al.
Analytical biochemistry, 225(1), 163-164 (1995-02-10)
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Protocols

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TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

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Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents

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