APREST71076

PrEST Antigen XRCC4

Prestige Antigens^™ Powered by Atlas Antibodies, buffered aqueous solution

• UNSPSC Code: 12352202

Properties

• recombinant: expressed in E. coli
• Assay: >80% (SDS-PAGE)
• form: buffered aqueous solution
• mol wt: predicted mol wt 33 kDa
• purified by: immobilized metal affinity chromatography (IMAC)
• concentration: ≥0.5 mg/mL
• immunogen sequence: KCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEK
• Ensembl | human accession no.: ENSG00000152422
• UniProt accession no.: Q13426
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... XRCC4(7518)

Description

General description

Recombinant protein fragment of Human XRCC4 with N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G) fusion tag. X-ray repair cross-complementing protein 4 (XRCC4) is also known as DNA repair protein XRCC4. It is mapped to human chromosome locus 5q14.2. It mediates joining of DNA double strand breaks during DNA repair.

Application

Suitable as a blocking agent using corresponding antibodies.

Biochem/physiol Actions

X-ray repair cross-complementing protein 4 (XRCC4) interacts with XRCC4-like factor (XLF) and DNA ligase IV in mediating nonhomologous end joining (NHEJ) cascade in DNA repair. A mutation in the XRCC4 is associated with adult-onset progressive encephalocardiomyopathy and ataxia. Patients with polymorphisms in XRCC4 gene are susceptible to schizophrenia. Single nucleotide polymorphisms in XRCC4 associates with high risk for cancer development. Mutations in XRCC4 leads to growth failure resulting in short stature accompanied by facial dysmorphism.(10)

Linkage

Corresponding Antibody HPA006801.

Physical form

Solution in 1 M urea-PBS, pH 7.4

Preparation Note

The protein solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Legal Information

Prestige Antigens is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable