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A8400

Sigma-Aldrich

Anti-Human Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD00162540
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:5,000-1:15,000 using IgG, IgA, IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Human immunoglobulins are glycoproteins that regulate immune responses to infections and allergies. Anti-human polyvalent immunoglobulins can be used for serological analyses in diseased population groups . These antibodies may also be used for studying xenotransplantation models and immunological disorders such as rheumatoid arthritis . Anti-Human Polyvalent Immunoglobulins (G,A,M)-Peroxidase antibody is specific for human IgG, IgA and IgM when tested against human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.

Immunogen

Purified human IgA, IgG, and IgM

Application

Anti-Human Polyvalent Immunoglobulins (G,A,M)-Peroxidase antibody is suitable for use in ELISA and immunoblot.
ELISA was performed to determine serotype-specific antibody avidity indexes for sera and monoclonal antibodies using HRP-conjugated goat anti-human polyvalent IgG with incubation for 2 hours.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ingeborg Kvivik et al.
Innate immunity, 27(4), 286-293 (2021-05-05)
Fatigue is common in all chronic inflammatory and autoimmune diseases. A conceptual model for understanding the biological basis of fatigue describes it as being a part of the sickness behaviour response generated by pro-inflammatory cytokines and other mediators. We hypothesised
J Nessa et al.
Journal of applied microbiology, 90(1), 68-72 (2001-01-13)
To use a commercial ELISA kit and an immunoblot assay to investigate the antibody levels of selected members of the Bangladeshi population to Helicobacter pylori protein antigens. Using immunoblotting, high seroprevalence rates were observed in all age groups, although the
Karl Albert Brokstad et al.
The Journal of infectious diseases, 185(7), 878-884 (2002-03-29)
The immune response in the nasal mucosa to influenza vaccination in 23 patients scheduled for tonsillectomy was studied. A statistically significant increase in influenza virus-specific serum and oral fluid antibodies was observed 7 days after vaccination. The numbers of influenza
H E Baxendale et al.
Infection and immunity, 74(2), 1025-1031 (2006-01-24)
Structure-function correlations of pneumococcal antibodies are important in predicting how changes in the pneumococcus (Pnc)-specific B-cell repertoire will influence humoral immunity against invasive Pnc disease. Using a unique panel of human hybridomas derived from memory B cells after pneumococcal conjugate
Béatrice Volney et al.
Acta tropica, 82(1), 11-23 (2002-03-21)
This paper describes a sero-epidemiological study of malaria prevalence in French Guiana. An immunofluorescence assay and an enzyme-linked immunosorbent assay were used to detect antibodies against blood-stage antigens and synthetic peptides mimicking the repetitive epitope of the sporozoites of Plasmodium
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