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41155

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® CAGE 500 antibody produced in goat

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

Synonym(s):

Abberior® CAGE 500P-Anti-Rabbit IgG antibody produced in goat

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.45

biological source

goat

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

availability

available only in USA and Canada

concentration

~1 mg/mL

fluorescence

λex 511 nm; λem 525 nm in PBS, pH 7.4 (photoactivated)

suitability

corresponds for SDS-PAGE

storage temp.

−20°C

General description

Abberior CAGE 500 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye which fluoresces at 511 nm.
Abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm, particularly as a 2 Color partner with CAGE 552 or FLIP 565.
Further, after photoactivation, Abberior CAGE 500 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities (e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore).


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (photoactivated, PBS pH 7.4), 299 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 50 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 525 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 590-610
Fluorescence Quantum Yield, λ: 0,85 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (photoactivated, PBS pH 7.4)

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior®

Legal Information

abberior is a registered trademark of Abberior GmbH

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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