The low or no protein recovery issue may be attributed to over-concentration of the protein, leading to precipitation. The User Guide provides guidance on maximizing sample recovery: Low sample recovery in the concentrate may be due to adsorptive losses, over-concentration, or passage of sample through the membrane. Adsorptive losses depend upon solute concentration, its hydrophobic nature, temperature and time of contact with filter device surfaces, sample composition, and pH. To minimize losses, remove concentrated samples immediately after centrifugal spin. If the starting sample concentration is high, monitor the centrifugation process in order to avoid over-concentration of the sample. Over-concentration can lead to precipitation and potential sample loss. If the sample appears to be passing through the membrane, choose a lower MWCO Amicon device.
UFC8003
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 3 kDa
Synonym(s):
Centrifugal concentrator, 3 kDa MWCO, 4 mL sample volume
Select a Size
Select a Size
About This Item
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Recommended Products
material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Ultra
parameter
4 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
pore size
3 kDa MWCO
shipped in
ambient
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
Application
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Physical form
Legal Information
Not finding the right product?
Try our Product Selector Tool.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
Filtration methods and membrane filter selection in nanoparticle purification and production.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Protocols
The human plasma proteome is a vast resource for biomarker discovery and investigation.
Related Content
Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.
-
What could be the reason for no protein recovery (in the concentrate or flow through) while using the UFC800324 to purify protein? Could the protein may be absorbed in the filter?
1 answer-
Helpful?
-
-
What is max g force these filters can take? i.e. what sort of rpm or g force should I spin at?
1 answer-
The UFC8003 protocol states to spin at 7,500 × g maximum for approximately 10–40 minutes. See the image below for further details.
Helpful?
-
-
Can we centrifuge these tubes in a normal 15 mL swinging bucket centrifuge?
1 answer-
Yes, the device can be used in centrifuges with a swinging bucket or fixed-angle rotor.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
