MABN755
Anti-TREM-2 Antibody, clone 78
clone 78, from rat
Synonym(s):
Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
78, monoclonal
species reactivity
mouse, human, rat
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TREM2(54209)
Related Categories
General description
TREM-2, also known as Triggering receptor expressed on myeloid cells 2 or Triggering receptor expressed on monocytes 2, and encoded by the gene Trem2/Trem2a/Trem2b/Trem2c, is a protein that forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. TREM-2 may also have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 is localized to the cell membrane and is a single pass membrane protein. TREM-2 is expressed in immune cells such as microglia and monocytes but the expression varies depending upon the exact tissue and location within the tissue. Defects in TREM-2 may be associated in humans with frontotemporal dementia, Nasu-Hakola disease, and recently an increase risk of Alzheimer′s disease.
Specificity
This antibody does not cross react with mouse TREM-1 or TREM-3 (Prof. William Seaman, University of California, San Francisco.).
Immunogen
Recombinant protein corresponding to mouse TREM-2.
Application
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected TREM-2 in TRACP+ osteoclasts (Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected TREM-2 in RAW264.7 cells (Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TREM-2 from mouse macrophage lysates (Peng, Q., et al. (2010). Sci Signal. 3(122):ra38.).
Inhibition Analysis: : Pre-treatment of BV2 cells or C57BL/6 Osteoclasts with a representative lot of this antibody significantly reduces cellular activation in response to both untreated and apoptotic Neuro2A cells (Hsieh, C. L., et al. (2009). J Neurochem. (109):4:1144-1156.; Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected TREM-2 in RAW264.7 cells (Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TREM-2 from mouse macrophage lysates (Peng, Q., et al. (2010). Sci Signal. 3(122):ra38.).
Inhibition Analysis: : Pre-treatment of BV2 cells or C57BL/6 Osteoclasts with a representative lot of this antibody significantly reduces cellular activation in response to both untreated and apoptotic Neuro2A cells (Hsieh, C. L., et al. (2009). J Neurochem. (109):4:1144-1156.; Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-TREM-2 Antibody, clone 78 is validated for use in western blotting, ICC, flow cytometry, inhibition & IP for the detection of TREM-2.
Quality
Evaluated by Western Blotting in human spleen tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TREM-2 in 10 µg of human spleen tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TREM-2 in 10 µg of human spleen tissue lysate.
Target description
~25 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Peng Zhao et al.
Molecular neurodegeneration, 17(1), 44-44 (2022-06-19)
Microglia plays crucial roles in Alzheimer's disease (AD) development. Triggering receptor expressed on myeloid cells 2 (TREM2) in association with DAP12 mediates signaling affecting microglia function. Here we study the negative regulation of TREM2 functions by leukocyte immunoglobulin-like receptor subfamily
Shih-Wei Chen et al.
Methods in molecular biology (Clifton, N.J.), 2683, 53-68 (2023-06-10)
The generation of a homogeneous population of microglia from human induced pluripotent stem cells (hiPSCs) is crucial to modeling neurological disorders, as well as the carrying out of drug screening and toxicity testing. Here, we provide a stepwise protocol for
Yingjun Zhao et al.
Neuron, 97(5), 1023-1031 (2018-03-09)
Mutations in triggering receptor expressed on myeloid cells 2 (TREM2) have been linked to increased Alzheimer's disease (AD) risk. Neurobiological functions of TREM2 and its pathophysiological ligands remain elusive. Here we found that TREM2 directly binds to β-amyloid (Aβ) oligomers
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