MABN1524

Anti-CHCHD10 Antibody, clone 11F11.2

clone 11F11.2, from mouse

• Synonym(s): Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial, Protein N27C7-4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 11F11.2, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001288268
• UniProt accession no.: Q8WYQ3
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CHCHD10(400916)

Description

General description

Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial (UniProt: Q8WYQ3; also known as Protein N27C7-4) is encoded by the CHCHD10 (also known as C22orf16) gene (Gene ID: 400916) in human. CHCHD10 belongs to a family of mitochondrial proteins located in the intermembrane space that is enriched at cristae junctions. CHCHD10 displays a ubiquitous distribution pattern, but its expression is highest in heart and liver and lowest in spleen. It is involved in the maintenance of mitochondrial organization, mitochondrial cristae structure, and mitochondrial DNA stability. CHCD10 has a non-structured N-terminal region, followed by a highly hydrophobic helix and a C-terminal CHCH domain characterized by a Cx(9)C motif and 2 additional cysteines. The four cysteine residues (102, 112, 122 and 132) of the CHCH domain are involved in two disulphide bonds. Overexpression of a CHCHD10 mutant allele in HeLa cells is reported to lead to fragmentation of the mitochondrial network and ultrastructural major abnormalities including loss, disorganization and dilatation of cristae. Mutations in CHCHD10 gene are reported to cause frontotemporal dementia and/or amyotrophic lateral sclerosis 2 (FTDALS2). (Ref.: Bannwarth, S., et al. (2014). Brain. 2014 137(Pt 8):2329-2345).

Specificity

Clone 11F11.2 detects CHCHD10 in human brain and liver.

Immunogen

GST-tagged recombinat fragment corresponding to 79 amino acids from human CHCHD10.

Application

Anti-CHCHD10 antibody, clone 11F11.2, Cat. No. MABN1524, is a highly specific mouse monoclonal antibody, that targets CHCHD10 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CHCHD10 in 10 µg of human liver tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHCHD10 in human small intestine, human tonsil, human liver, and human prostate cancer tissue.

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CHCHD10 in 10 µg of human brain tissue lysate.

Target description

~15 kDa observed; 14.15 kDa calulated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1