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MABF169C

Sigma-Aldrich

Anti-CD8a (human), redFluor® 710, clone RPA-T8 Antibody

clone RPA-T8, from mouse, redFluor® 710

Synonym(s):

T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD antigen CD8a, CD8a

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

redFluor® 710

antibody form

purified antibody

antibody product type

primary antibodies

clone

RPA-T8, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... CD8A(925)

General description

The RPA-T8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, Hit8a, the RPA-T8 antibody will not block binding of Hit8a to CD8a.

Application

This mouse monoclonal Anti-CD8a Antibody (human), redFluor® 710, clone RPA-T8, Cat. No. MABF16965 is validated for use in Flow Cytometry, for the detection of CD8a.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD8a in one million human peripheral blood lymphocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

redFluor is a registered trademark of Tonbo Biotechnologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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