HTS087RTA
Ready-to-Assay α 1A Adrenergic Receptor Frozen Cells
Human
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.76
Recommended Products
General description
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
The endogenous catecholamines, epinephrine and norepinephrine have profound effects on smooth muscle activity, cardiac function, carbohydrate and fat metabolism, hormone secretion, neurotransmitter release, and central nervous system actions. These activities are mediated by GPCRs belonging to two subfamilies, the - and -adrenoceptors (Bylund et al., 1994). The three members of the α1 subclass of adrenoceptors, α1A, α1B and α1D, couple to Gq, and promote contraction of vascular and urinary tract smooth muscle, relaxation of intestinal smooth muscle, increased contractile force in the heart, and glycogenolysis and gluconeogenesis in the liver. The different subtypes have overlapping distributions and variably contribute to these effects depending on species and tissue; the α1A subtype plays a prominent role in urogenital smooth muscle contraction and renal artery contraction (Hrometz et al., 1999; Ruffolo and Hieble, 1999). Activation of α1 adrenoceptors also influences cell proliferation; α1A inhibits cell growth by arresting progression at the G1/S transition (Shibata et al., 2003). The α1A subtype undergoes alternative splicing to generate four variants that differ at their C-termini, although these variants appear to be functionally identical (Chang et al., 1998). Millipore′s cloned human α1A -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant α1A expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening agonists, antagonists and modulators at α1A adrenergic receptors.
The endogenous catecholamines, epinephrine and norepinephrine have profound effects on smooth muscle activity, cardiac function, carbohydrate and fat metabolism, hormone secretion, neurotransmitter release, and central nervous system actions. These activities are mediated by GPCRs belonging to two subfamilies, the - and -adrenoceptors (Bylund et al., 1994). The three members of the α1 subclass of adrenoceptors, α1A, α1B and α1D, couple to Gq, and promote contraction of vascular and urinary tract smooth muscle, relaxation of intestinal smooth muscle, increased contractile force in the heart, and glycogenolysis and gluconeogenesis in the liver. The different subtypes have overlapping distributions and variably contribute to these effects depending on species and tissue; the α1A subtype plays a prominent role in urogenital smooth muscle contraction and renal artery contraction (Hrometz et al., 1999; Ruffolo and Hieble, 1999). Activation of α1 adrenoceptors also influences cell proliferation; α1A inhibits cell growth by arresting progression at the G1/S transition (Shibata et al., 2003). The α1A subtype undergoes alternative splicing to generate four variants that differ at their C-termini, although these variants appear to be functionally identical (Chang et al., 1998). Millipore′s cloned human α1A -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant α1A expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening agonists, antagonists and modulators at α1A adrenergic receptors.
Cell Line Description
- GPCR Cell Lines
- Host cells: Chem-1
Application
Human alpha1A GPCR frozen cells for Calcium Flux FLIPR Assays.
Biochem/physiol Actions
- GPCR Class: A
- Protein Target: alpha1A
- Target Sub-Family: Adrenergic
Components
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Disclaimer
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service