Purified mouse monoclonal antibody. Recognizes the ~120 kDa TLR7 protein.
Recognizes the ~120 kDa TLR7 protein in IMR32 cells.
This Anti-TLR7 Mouse mAb (2C9) is validated for use in ELISA, Immunoblotting for the detection of TLR7.
Immunogen
A recombinant polypeptide corresponding to amino acids of 27-127 human TLR7, expressed as a GST fusion protein
Application
ELISA (see comments) Immunoblotting (1-5 g/ml)
Warning
Toxicity: Regulatory Review (Z)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control IMR32 cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Medical science monitor : international medical journal of experimental and clinical research, 26, e923104-e923104 (2020-05-27)
BACKGROUND The metabolic processing of ellagic acid (EA) by cytochrome P450s (CYP450s) expressed in the intestines is unclear. This study aimed to investigate the effects of CYP450s that are highly expressed in HIEC cells on metabolic activity of EA. MATERIAL
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