ABE416

Anti-SHMT1 Antibody

from sheep, purified by affinity chromatography

• Synonym(s): Serine hydroxymethyltransferase, cytosolic, SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: sheep
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• technique(s): ChIP: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_033197
• UniProt accession no.: P50431
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SHMT1(6470)

Description

General description

Serine hydroxymethyltransferase (SHMT) is also known as glycine hydroxymethyltransferase or serine methylase, and is an enzyme that catalyzes the reversible conversion of serine and glycine. SHMT is thought to be involved in the epigenetic DNA methylation mechanism and has shown increased activity during cell proliferation and the S phase of the cell cycle. Evidence indicates that SHMT repression is a critical step in the Retinoic Acid-induced cell growth arrest and differentiation pathway.

Immunogen

KLH-conjugated linear peptide corresponding to mouse SHMT1.

Application

Anti-SHMT1 Antibody is a highly specific sheep polyclonal antibody, that targets Serine hydroxymethyltransferase & has been tested in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of mouse liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).

Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected SHMT1 when coprecipitated with PCNA in pBABE-Puro-SV50-LT transfected HeLa nuclear extracts. MacFarlane A.J., et al. (2011). J Biol Chem. 286(51):44015-22

Quality

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of human liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).

Target description

~53 kDa observed. An uncharacterized band may be observed at ~40 kDa in some tissue lysates.

Physical form

Affinity purified

Purified sheep polyclonal in buffer containing 0.5M HEPES with 0.05% sodium azide and 50% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Human liver tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1