ABE125

Anti-PRMT7 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Protein arginine N-methyltransferase 7, Histone-arginine N-methyltransferase PRMT7, Myelin basic protein]-arginine N-methyltransferase PRMT7
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): feline (based on 100% sequence homology), bovine (based on 100% sequence homology), ferret (based on 100% sequence homology), horse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_061896
• UniProt accession no.: Q9NVM4
• shipped in: wet ice

Description

General description

PRMT7, also known as Protein arginine N-methyltransferase 7, Histone-arginine N-methyltransferase PRMT7 or Myelin basic protein-arginine N-methyltransferase PRMT7, and encoded by the gene PRMT7/KIAA1933, is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT7 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins SmD1 (SNRPD1) and SmD3 (SNRPD3). Such methylation is required for the assembly and biogenesis of snRNP core particles. PRMT7 also mediates the symmetric dimethylation of histone H4 ′Arg-3′ to form H4R3me2s, and it plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s and thus leading to the recruitment of DNA methyltransferases to those sites. Finally, PRMT7 may also play a role in embryonic stem cell (ESC) pluripotency and is able to methylate histone H2A and myelin basic protein (MBP).

Specificity

This antibody is expected to cross react with all isoforms based upon immunogen design.

Immunogen

Epitope: unknown

GST-tagged recombinant protein corresponding to human PRMT7

Application

Anti-PRMT7 Antibody detects level of PRMT7 & has been published & validated for use in PRMT7.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in U2OS nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PRMT7 in 10 µg of U2OS nuclear extract.

Target description

~75 kDa observed. Uniprot describes four isoforms at 79 kDa (Isoform 1), 62 kDa (Isoform 2), 73 kDa (Isoform 3), and 64 kDa (Isoform 4).

Physical form

Affinity purified

Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13