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1.10987

Millipore

Phenol-red broth (base)

suitable for microbiology

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500 G
€234.00

€234.00

Estimated to ship on23 April 2025

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500 G
€234.00

About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

€234.00

Estimated to ship on23 April 2025

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form

medium granules (dehydrated (DCM))

Quality Level

200

manufacturer/tradename

GranuCult®

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

pH

7.2-7.6 (30 °C, 15 g/L in H2O, after autoclaving)

solubility

15 g/L

bulk density

650 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

bacteria

Analysis Note

Appearance (clearness): clear
Appearance (colour): red
pH-value (25 °C): 7.2 - 7.6
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): good to very good
Growth (Enterococcus faecalis ATCC 11700): good to very good
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Citrobacter freundii ATCC 8090): good to very good
Growth (Proteus hauseri ATCC 13315): good to very good
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Colour change to yellow (Staphylococcus aureus ATCC 25923 (WDCM 00034)): +
Colour change to yellow (Enterococcus faecalis ATCC 11700): +
Colour change to yellow (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Colour change to yellow (Citrobacter freundii ATCC 8090): +
Colour change to yellow (Proteus hauseri ATCC 13315): +
Colour change to yellow (Shigella flexneri ATCC 12022 (WDCM 00126)): +/- (orange)
Colour change to yellow (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Gas (Staphylococcus aureus ATCC 25923 (WDCM 00034)): -
Gas (Enterococcus faecalis ATCC 11700): -
Gas (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Gas (Citrobacter freundii ATCC 8090): +
Gas (Proteus hauseri ATCC 13315): + (poor)
Gas (Shigella flexneri ATCC 12022 (WDCM 00126)): -
Gas (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Incubation: 24 hrs.; 35 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

New EN ISO 22964 for Cronobacter spp. detection in the food chain

The new EN ISO 22964:2017 standard specifies a horizontal method for the detection of Cronobacter spp. in food, animal feed and environmental samples.

Questions

  1. Good day Does this product "1.10987 Phenol-red broth (base)" require supplements when preparing a solution for microbe growth? And if so what supplements are needing.

    1 answer

    1. Phenol-red broth (base) 1.10987 is used to evaluate the ability of micro-organisms to cause fermentation of a given reactant. The datasheet below mentions all the reactants that can be used in conjunction with this medium to perform the test:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/311/079/386-phenol-red-broth-110987-mk.pdf

      Additionally phenol red can be added for a better visualization of the pH change during reaction. The corresponding reference is also mentioned in the datasheet.
      Also note that in case of anaerobic conditions, it is sometimes best to also add as another supplement agar-agar, again referred to in the documentation.

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