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Sigma-Aldrich

Dihydrofluorescein diacetate

97%

Synonym(s):

Diacetyldihydrofluorescein

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About This Item

Empirical Formula (Hill Notation):
C24H18O7
CAS Number:
35340-49-9
Molecular Weight:
418.40
Beilstein:
364167
MDL number:
MFCD00005056
UNSPSC Code:
12171500
PubChem Substance ID:
24857603
NACRES:
NA.47

Assay

97%

mp

213-215 °C (lit.)

solubility

acetone: 50 mg/mL

fluorescence

λex 272 nm in methanol
λex 490; λem 514 (Reaction product)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(=O)Oc1ccc2C(c3ccc(OC(C)=O)cc3Oc2c1)c4ccccc4C(O)=O

InChI

1S/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28)

InChI key

YKSJJXGQHSESKB-UHFFFAOYSA-N

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General description

Dihydrofluorescein diacetate (H2FDA) is an esterified derivative of dihydrofluorescein. H2FDA is one of the widely used fluorescent probes employed in the intracellular reactive oxygen species (ROS) detection.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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