SeQuant^® HILIC HPLC Columns

Section Overview

• Complete Range for Separations of All Polar Hydrophilic Compounds
• SeQuant^® ZIC^®-HILIC
• SeQuant^® ZIC^®-cHILIC
• SeQuant^® ZIC^®-pHILIC

Complete Range for Separations of All Polar Hydrophilic Compounds

SeQuant^® HILIC (hydrophilic interaction liquid chromatography) HPLC columns constitute a range of high-performance tools for separating polar hydrophilic compounds. All columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. Separation is achieved by hydrophilic partitioning combined with weak ionic interactions for maximum selectivity, high loadability and easy optimization of methods.

Features and Benefits

• High-performance HPLC and LC-MS separations of polar hydrophilic compounds
• Zwitterionic stationary phase ensures reproducible retention
• Two complementary phase chemistries
• Maximum LC-MS compatibility with minimized column bleed
• Excellent reproducibility and robustness
• Available in a variety of lengths, particle sizes, and pore dimensions

Choose Your SeQuant® HILIC Selectivity
SeQuant® Column	Functional Group	Features	Base Particle	Particle Size	Pore Size	Column Types
SeQuant® ZIC®-HILIC	Sulfobetaine	High-performance selectivity and robustness	silica	3.5 µm, 5 µm	100 Å, 200 Å	analytical, capillary, semi-prep, guards
SeQuant® ZIC®-cHILIC	Phosphory-lcholine	Complementary selectivity with favorable LC-MS performance	silica	3 µm	100 Å	analytical, capillary, guards
SeQuant® ZIC®-pHILIC	Sulfobetaine	High pH-stability and low noise with ion detectors	polymer	5 µm		analytical, guards

SeQuant^® ZIC^®-HILIC

The ZIC^®-HILIC Column is designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) is affected during eluent optimization.

SeQuant^® ZIC^®-cHILIC

SeQuant^® ZIC^®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This new zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.

Isocratic separations of the positively charged benzyltrimethylamine (BTMA, peaks indicated with arrows) and the neutral toluene (void marker), uracil and cytosine on ZIC^®-cHILIC (left) and ZIC^®-HILIC (right) illustrating differences and similarities in selectivity caused by the different charge orientation of the zwitterionic functional groups (see illustrations). Column dimensions were 100 x 4.6 mM, particles size 3 or 3.5 µm, and pore size 100 Å. Eluent was 80 : 20 acetonitrile/ 25 mM aqueous ammonium acetate pH 6.8 pumped at 0.5 mL/min at 23 °C. Detection by UV absorption at 254 nm.

SeQuant^® ZIC^®-pHILIC

The ZIC^®-pHILIC stationary phase has the same highly polar, bonded, permanent zwitterion, functional group as the silica based ZIC^®-HILIC Column. The user can therefore expect the same selectivity, however, with a trade-off in flow-rate range, and separation efficiency, common with polymeric materials. The more durable support allows for use in an extended pH range, which can be beneficial for certain applications.

Example of enhanced selectivity at high pH The application example below illustrates how the selectivity of the ZIC^®-pHILIC material can be enhanced by performing the separation at elevated pH. The chromatograms show isocratic separations of gentisic acid, protocatechuic acid and isophthalic acid on a ZIC^®-pHILIC Column. The pH-increase also results in higher retention and improved peak shape for these analytes.

Ordering Information
SeQuant	Length (mm)	ID (mm)	ZIC®-HILIC 200A, 5 µm	ZIC®-HILIC 100A, 3.5 µm	ZIC®-HILIC 200A, 3.5 µm	ZIC®-cHILIC 100A, 3µm	ZIC®-pHILIC, 5µm
HPLC Column	50	2.1	1.50450	1.50440	1.50445	1.50656	1.50459
HPLC Column	50	4.6	1.50451		1.50446	1.50659	1.50463
HPLC Column	50	21.2	1.50496
HPLC Column	100	2.1	1.50452	1.50441	1.50447	1.50657	1.50462
HPLC Column	100	4.6	1.50453			1.50660	1.50464
HPLC Column	150	2.1	1.50454	1.50442	1.50448	1.50658	1.50460
HPLC Column	150	4.6	1.50455	1.50444	1.50449	1.50661	1.50461
HPLC Column	150	10	1.50493
HPLC Column	150	21.2	1.50497
HPLC Column	250	2.1	1.50457	1.50443
HPLC Column	250	4.6	1.50458			1.50662
HPLC Column	250	10	1.50494
Guard column	20	2.1	1.50435	1.50439		1.50664	1.50437
Guard Kit	20	2.1					1.50438
Capillary column	30	0.3			1.50489
Capillary column	30	1			1.50478
Capillary column	150	0.075	1.50465
Capillary column	150	0.3	1.50481		1.50479	1.50669
Capillary column	150	1	1.50482	1.50487	1.50480	1.50670
Guard capillary	5	0.3	1.50492			1.50665
Guard capillary	5	1	1.50490			1.50666
Guard Fitting	14	1	1.50434
Guard Kit	20	1	1.50436