The histidine kinase CusS senses silver ions through direct binding by its sensor domain.

The histidine kinase CusS senses silver ions through direct binding by its sensor domain.

Biochimica et biophysica acta (2014-06-21)

Swapna A Gudipaty, Megan M McEvoy

ZUSAMMENFASSUNG

The Cus system of Escherichia coli aids in protection of cells from high concentrations of Ag(I) and Cu(I). The histidine kinase CusS of the CusRS two-component system functions as a Ag(I)/Cu(I)-responsive sensor kinase and is essential for induction of the genes encoding the CusCFBA efflux pump. In this study, we have examined the molecular features of the sensor domain of CusS in order to understand how a metal-responsive histidine kinase senses specific metal ions. We find that the predicted periplasmic sensor domain of CusS directly interacts with Ag(I) ions and undergoes a conformational change upon metal binding. Metal binding also enhances the tendency of the domain to dimerize. These findings suggest a model for activation of the histidine kinase through metal binding events in the periplasmic sensor domain.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

735825

Sigma-Aldrich

Silber, conductive paste

203122

Sigma-Aldrich

Kupfer, powder, 99.999% trace metals basis

576832

Sigma-Aldrich

Silber, nanopowder, <100 nm particle size, contains PVP as dispersant, 99.5% trace metals basis

349178

Sigma-Aldrich

Kupfer, foil, thickness 0.25 mm, 99.98% trace metals basis

266086

Sigma-Aldrich

Kupfer, powder, <425 μm, 99.5% trace metals basis

327085

Sigma-Aldrich

Silber, powder, 2-3.5 μm, ≥99.9% trace metals basis

349216

Sigma-Aldrich

Kupfer, wire, diam. 1.0 mm, ≥99.9%

327077

Sigma-Aldrich

Silber, flakes, 10 μm, ≥99.9% trace metals basis

207780

Sigma-Aldrich

Kupfer, powder, <75 μm, 99%

739421

Sigma-Aldrich

Silber-Nanodrähte, diam. × L 50 nm × 6 μm, 0.5% (isopropyl alcohol suspension)

484059

Sigma-Aldrich

Silber, nanopowder, <150 nm particle size, 99% trace metals basis

774111

Sigma-Aldrich

Kupfer, nanopowder, 40-60 nm particle size (SAXS), ≥99.5% trace metals basis

326453

Sigma-Aldrich

Kupfer, powder (spheroidal), 10-25 μm, 98%

327026

Sigma-Aldrich

Silber, wire, diam. 0.5 mm, 99.9% trace metals basis

778095

Sigma-Aldrich

Silber-Nanodrähte, diam. × L 200 nm × 10 μm, 0.5% (isopropyl alcohol suspension)

326429

Sigma-Aldrich

Kupfer, wire, diam. 0.25 mm, 99.999% trace metals basis

327093

Sigma-Aldrich

Silber, powder, 5-8 μm, ≥99.9% trace metals basis

774103

Sigma-Aldrich

Kupfer, nanopowder, 60-80 nm particle size (SAXS), ≥99.5% trace metals basis

349208

Sigma-Aldrich

Kupfer, foil, thickness 0.025 mm, 99.98% trace metals basis

61139

Sigma-Aldrich

Kupfer, turnings, purum p.a., ≥99.0%

12816

Sigma-Aldrich

Kupfer, foil, ≥99.8% (complexometric)

265527

Sigma-Aldrich

Silber, foil, thickness 0.1 mm, 99.9% trace metals basis

357456

Sigma-Aldrich

Kupfer, powder (dendritic), <45 μm, 99.7% trace metals basis

326410

Sigma-Aldrich

Kupfer, wire, diam. 0.64 mm, 99.995% trace metals basis

349151

Sigma-Aldrich

Kupfer, foil, thickness 0.5 mm, 99.98% trace metals basis

327107

Sigma-Aldrich

Silber, powder, <45 μm, ≥99.99% trace metals basis

85131

Sigma-Aldrich

Silber, colloidal, 65-75% Ag basis

739448

Sigma-Aldrich

Silber-Nanodrähte, diam. × L 100 nm × 6 μm, 0.5% (isopropyl alcohol suspension)

265608

Sigma-Aldrich

Silber, wire, diam. 1.0 mm, 99.9% trace metals basis

327050

Sigma-Aldrich

Silber, shot, 1-3 mm, ≥99.99% trace metals basis