Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis.

Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis.

Autophagy (2015-09-24)

Mandy Oj Grootaert, Paula A da Costa Martins, Nicole Bitsch, Isabel Pintelon, Guido Ry De Meyer, Wim Martinet, Dorien M Schrijvers

PMID26391655

ZUSAMMENFASSUNG

Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its significance in the development of postinjury neointima formation and atherosclerosis. Tissue-specific deletion of the essential autophagy gene Atg7 in murine VSMCs (atg7

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

A5441

Sigma-Aldrich

Monoklonales Anti-β-Aktin in Maus hergestellte Antikörper, clone AC-15, ascites fluid

A2547

Sigma-Aldrich

Monoklonaler Anti-Aktin-Antikörper , α-Glattmuskel, clone 1A4, ascites fluid

CS0030

Sigma-Aldrich

Histochemisches Färbungskit für Seneszenzzellen, sufficient for 100 tests

F3777

Sigma-Aldrich

Monoklonaler Anti-Aktin α-Glattmuskel-FITC-Antikörper der Maus in Maus hergestellte Antikörper, clone 1A4, purified from hybridoma cell culture

P0067

Sigma-Aldrich

Anti-p62/SQSTM1 in Kaninchen hergestellte Antikörper, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

A2856

Sigma-Aldrich

Anti-ATG7 in Kaninchen hergestellte Antikörper, affinity isolated antibody, buffered aqueous solution

A0856

Sigma-Aldrich

Anti-ATG5 (N-terminal) in Kaninchen hergestellte Antikörper, affinity isolated antibody, PBS solution

SAB4503014

Sigma-Aldrich

Anti-Acetyl (Lys 319) p53 Antibody, C-Terminal antibody produced in rabbit, affinity isolated antibody