84-109

HPLC Analysis of Triazine Herbicides in Grass on SUPELCOSIL^™ LC-8-DB after SPE using Supelclean^™ LC-SCX

application for SPE, application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable; solid phase extraction (SPE): suitable
• Testparameter: sample preparation: SPE (Solid Phase Extraction); sample/matrix: fresh grass clippings spiked with herbicides at 2 ppm; SPE tube/cartridge: Supelclean LC-SCX, 500 mg/3 mL (57018); condition: 1 mL methylene chloride; sample addition: 2 mL grass extract. Wash with 2 x 2 mL acetonitrile.; drying: Dry packing for 5 min. under nitrogen purge; washing: Dry packing for 5 min under nitrogen purge; wash with 2 x 2 mL DI water; elution: 1.5 mL methanol; eluate post-treatment: dilute to 2 mL with DI water; column: SUPELCOSIL LC-8-DB, 15 cm × 4.6 mm I.D., 5 μm (58347-U) with guard column (59553); mobile phase: [A] acetonitrile; [B] water; (45:55, A:B); flow rate: 1.5 mL/min; detector: UV, 254 nm; injection: 100 μL
• Eignung: application for HPLC; application for SPE
• Anwendung(en): environmental

Beschreibung

Anwendung

Sample Pre-treatment:
5 g fresh grass clippings spiked with 2 ppm each herbicide. Add 4 g anhydrous sodium sulfate and 20 mL methylene chloride:acetone (80:20). Shake 20 min and allow mixture to stand 1 min.

Rechtliche Hinweise

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analyt

Atrazine
Simazine
Propazine