ZRB2334

Anti-DYNLL1/PIN Antibody, clone 4B19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): 8 kDa dynein light chain, DLC8, Dynein light chain 1, cytoplasmic, Dynein light chain LC8-type 1, PIN, Protein inhibitor of neuronal nitric oxide synthase
• UNSPSC-Code: 12352203
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 4B19, recombinant monoclonal
• Beschreibung: recombinant, expressed in HEK 293 cells
• Produktlinie: ZooMAb^® learn more
• Form: lyophilized
• Mol-Gew.: calculated mol wt 10.37 kDa; observed mol wt ~24 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): rat, mouse
• Verpackung: antibody small pack of 25 μL
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Methode(n): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG
• Epitopsequenz: N-terminal half
• Protein-ID-Hinterlegungsnummer: NP_003737
• UniProt-Hinterlegungsnummer: P63167
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... DYNLL1(8655)

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 4B19 ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects DYNLL1. It targets an epitope within 13 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal half of human DYLL1.

Anwendung

Quality Control Testing

Evaluated by Western Blotting with His/ABP-tagged recombinant fragment of Human DYNLL1.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His/ABP-tagged recombinant fragment of Human DYNLL1.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DYNLL1/PIN in Human liver tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected DYNLL1/PIN in MCF-7 cells.

Affinity Binding Assay: A representative lot of this antibody bound recombinant fragment of DYNLL1/PIN with a KD of 2.8 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Dynein light chain 1, cytoplasmic (UniProt: P63167; also known as 8 kDa dynein light chain, DLC8, Dynein light chain LC8-type 1, Protein inhibitor of neuronal nitric oxide synthase, PIN) is encoded by the DYNLL1 (also known as DLC1, DNCL1, DNCLC1, HDLC1) gene (Gene ID: 8655) in human. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains, and a number of non-catalytic subunits presented by intermediate chains, light intermediate chains, and light chains.  The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The intermediate and light intermediate chains bind directly to the heavy chain dimer and the light chains assemble on the intermediate dimer. Dynein light chain 1 (DYNLL1) is a ubiquitously expressed protein that can exist either as a homodimer or as a monomer. The monomeric form is shown to be incapable of binding to its target proteins. The transition from monomer to dimer is crucial for its function, and this process is regulated by p21-activated kinase 1 phosphorylation of serine 88. It acts as one of the several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins to regulate dynein function. Its expression is observed in cytoplasm, nucleus, and mitochondria. Cytoplasmic DYNLL1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules and may play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its anti-apoptotic activity. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Valdamudi, RK., et al. (2004). Cancer Cell. 5(6); 575-585; Puthalakath, H., et al. (1999). Mol. Cell. 3(3); 287-296).

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstituierung

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable