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Z0276

Sigma-Aldrich

Anti-phospho-ZAP-70 (pTyr315/pTyr319) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL-Nummer:
MFCD07370447
UNSPSC-Code:
12352203

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous glycerol solution

Speziesreaktivität

human, mouse

Methode(n)

western blot: 1:1,000 using Jurkat cells treated with H2O2.

UniProt-Hinterlegungsnummer

P43403

Versandbedingung

dry ice

Lagertemp.

−20°C

Angaben zum Gen

human ... ZAP70(7535)
mouse ... Zap70(22637)

Allgemeine Beschreibung

ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the ζ chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling. Phosphorylation of Tyr315 and Tyr319 residues regulates TCR-mediated signal transduction . Anti-phospho-ZAP-70 pTyr315/pTyr319) antibody is specific for human ZAP-70. The product is expected to react with mouse ZAP-70 (as its 100% homologous to human ZAP-70), but has not been tested for it.

Immunogen

synthetic phosphopeptide derived from the region of human ZAP-70 that contains tyrosines 315 and 319.

Anwendung

Anti-phospho-ZAP-70 pTyr315/pTyr319) antibody is suitable for use in western blot (1:1,000 using Jurkat cells treated with H2O2 and immunoblot.

Physikalische Form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

B L Williams et al.
The EMBO journal, 18(7), 1832-1844 (1999-04-15)
Accumulating evidence indicates that the interdomain B regions of ZAP-70 and Syk play pivotal roles in the coupling of T-cell antigen receptor (TCR) stimulation to the activation of downstream signaling pathways. The interdomain B region of ZAP-70 contains at least
V Di Bartolo et al.
The Journal of biological chemistry, 274(10), 6285-6294 (1999-02-26)
Following T cell antigen receptor (TCR) engagement, the protein tyrosine kinase (PTK) ZAP-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family PTK Lck. These events have been implicated in
A C Chan et al.
Cell, 71(4), 649-662 (1992-11-13)
Protein-tyrosine kinases (PTKs) play an integral role in T cell activation. Stimulation of the T cell antigen receptor (TCR) results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR zeta chain, which can mediate

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