T2824

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster

~2 mg/mL, clone TN3-19.12, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-TNF-α
• MDL-Nummer: MFCD01632994
• UNSPSC-Code: 51111800
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: hamster
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: TN3-19.12, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: rat, mouse
• Verpackung: antibody small pack of 25 μL
• Konzentration: ~2 mg/mL
• Methode(n): immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; neutralization: suitable; western blot: 0.25-0.5 μg/mL using recombinant mouse TNF-α
• UniProt-Hinterlegungsnummer: P16599
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Tnf(21926) ; rat ... Tnf(24835)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.

Spezifität

Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.

Immunogen

purified recombinant mouse TNF-α.

Anwendung

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster has been used in:

• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• neutralization

Biochem./physiol. Wirkung

TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.

Physikalische Form

Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable