T0678

Anti-Tryptophan Hydroxylase Antibody

mouse monoclonal, WH-3

• Synonym(e): Anti-TPH
• MDL-Nummer: MFCD00282500
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Produktbezeichnung: Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, clone WH-3, ascites fluid
• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: primary antibodies
• Klon: WH-3, monoclonal
• Mol-Gew.: antigen 55 kDa
• Enthält: 15 mM sodium azide
• Speziesreaktivität: human, monkey, rabbit, rat
• Methode(n): immunohistochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1:1,000 using rabbit pineal gland extract
• Isotyp: IgG3
• UniProt-Hinterlegungsnummer: P17752
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... TPH1(7166) , TPH2(121278) ; rat ... Tph1(24848) , Tph2(317675)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; L-tryptophan tetrahydropteridine:oxygen oxidoreductase; EC 1.14.16.4), the rate-limiting enzyme for the synthesis of serotonin, is a very specific enzyme found only inside serotoninergic neurons.

The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland.

Spezifität

This antibody reacts specifically with tryptophan hydroxylase.

Immunogen

recombinant rabbit tryptophan hydroxylase.

Anwendung

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry
• immunoblot
• immunocytochemistry

Biochem./physiol. Wirkung

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable