SRP6263

PLGF/PIGF2/PGF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(e): PGFL, SHGC-10760
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in HEK 293 cells
• Markierung: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol-Gew.: calculated mol wt 20.2 kDa; observed mol wt 28-33 kDa (DTT-reduced. Protein migrates due to glycosylation. Arg 29 is the predicted N-terminus.)
• Verpackung: pkg of 10 μg
• Verunreinigungen: <1 EU/μg endotoxin (LAL test)
• UniProt-Hinterlegungsnummer: P49763
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... PLGF(5228)

Beschreibung

Allgemeine Beschreibung

Placental growth factor (PLGF) exists in two isoforms namely PLGF1 and PLGF2. This protein is a homodimeric glycoprotein with molecular weight of 46–50kDa and is a member of the VEGF (vascular endothelial growth factor) subfamily. PLGF is encoded by the gene mapped to human chromosome 14q24-q31 regions. PLGF is expressed mainly in the placental trophoblast during pregnancy and is also present in various other tissues such as the villous trophoblast.

Biochem./physiol. Wirkung

PLGF might enhance the production of peripheral blood mononuclear cell-vascular endothelial growth factor (PBMC-VEGF). Consequently, PlGF might facilitate VEGF production in the inflammatory joint. Soluble fms related tyrosine kinase 1 (Flt-1), which is considered to be an anti-angiogenic factor interacts with and inhibit the activity of PLGF. Therefore, PLGF level is lowered and the Flt-1 level is increased in placental tissue of women with pre-eclampsia (PE). PLGF is associated with the pathogenesis of rheumatoid arthritis (RA) and various other arthropathies. PLGF acts as a ligand for VEGFR-1 and is implicated in various physiological functions such as tumor angiogenesis, wound healing and hematopoietic progenitors recruitment.

Physikalische Form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable