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Merck

SAB5801060

Sigma-Aldrich

Goat Anti-Rabbit IgG(Fcγ) HRP Alpaca Heavy chain only Antibody

recombinant Alpaca(VHH) Nano antibody

Synonym(e):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

alpaca

Rekombinant

expressed in HEK 293 cells (Human cell line)

Konjugat

horse radish peroxidase (HRP)

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

secondary antibodies

Klon

recombinant monoclonal

Form

lyophilized solid

Mol-Gew.

100 kDa

Speziesreaktivität

rabbit, Alpaca

Methode(n)

ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000

Größe

0.1 mL

Farbe

colorless

Isotyp

VHH

Versandbedingung

wet ice

Lagertemp.

2-8°C

Allgemeine Beschreibung

Goat Anti-Rabbit IgG(Fcγ) HRP Alpaca Heavy chain only Antibody is designed to specifically detect Fc region of rabbit IgG. It is a monoclonal, recombinant, goat IgG Fc fused single domain antibody to Fc region of rabbit IgG coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Goat Anti-Rabbit IgG(Fcγ) HRP Alpaca Heavy chain only Antibody reacts with the Fc fragment of rabbit IgG selectively but not with mouse, human, cynomolgus, rat, goat IgG.
Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Spezifität

Fc region of Rabbit IgG
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Verpackung

100 μg in Glass bottle

Physikalische Form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Rekonstituierung

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Lagerung und Haltbarkeit

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

Sonstige Hinweise

No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG

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Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Aquatic Chronic 3 - Skin Sens. 1

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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