SAB4702127

Monoclonal Anti-CD22 PE-Cyanine7 produced in mouse

• Synonym(e): SIGLEC-2, SIGLEC2
• UNSPSC-Code: 12352203

Eigenschaften

• Konjugat: PE-Cy7 conjugate
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: S-HCL-1, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: human
• Methode(n): flow cytometry: suitable
• Isotyp: IgG2bκ
• UniProt-Hinterlegungsnummer: P20273
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CD22(933)

Beschreibung

Allgemeine Beschreibung

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Spezifität

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

Immunogen

Whole hairy cell leukemia cells and membrane preparation

Physikalische Form

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Angaben zur Herstellung

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Lagerung und Haltbarkeit

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Sonstige Hinweise

The antibody solution should be gently mixed before use.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable