SAB4702103

Monoclonal Anti-Neurofilament H, purified produced in mouse

• Synonym(e): CMT2CC, NEFH, NFH, Neurofilament heavy chain
• UNSPSC-Code: 12352203

Eigenschaften

• Konjugat: unconjugated
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: NF-01, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: (Mammalian)
• Konzentration: 1 mg/ml
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P12036
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NEFH(4744)

Beschreibung

Allgemeine Beschreibung

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.

Spezifität

The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Immunogen

Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.

Physikalische Form

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Angaben zur Herstellung

Purified by protein-A affinity chromatography.

Lagerung und Haltbarkeit

Store at 2-8°C. Do not freeze.

Sonstige Hinweise

The antibody solution should be gently mixed before use.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable