SAB4500028

Anti-AOS1 antibody produced in rabbit

affinity isolated antibody

• Synonym(e): AOS1, SAE1, SUA1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A
• MDL-Nummer: MFCD03454733
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 38 kDa
• Speziesreaktivität: rat, mouse, human
• Konzentration: ~1 mg/mL
• Methode(n): ELISA: 1:20000; immunofluorescence: 1:100-1:500; western blot: 1:500-1:1000
• NCBI-Hinterlegungsnummer: Q9UBE0
• UniProt-Hinterlegungsnummer: Q9UBE0
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SAE1(10055)

Beschreibung

Allgemeine Beschreibung

E1 ubiquitin-activating protein AOS1 also known as SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1) is encoded by the gene mapped to human chromosome 19. Human ASO1 is expressed throughout nuclei apart from nucleoli.The protein expression is elevated in S phase and decreased in G2 phase of cell cycle.

Immunogen

The antiserum was produced against synthesized peptide derived from human AOS1.

Immunogen Range: 221-270

Biochem./physiol. Wirkung

SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1)/ AOS1 transcription is induced by Myc via direct binding to canonical E-Boxes sequences present near the SAE1 transcription start site. SAE1 and SAE2/ UBA2 complex plays a crucial role in the tumorigenicity of Myc-dependent cancer cells in vitro and in vivo. SAE1/SAE2 heterodimer complex catalyzes the conjugation of SUMO-1 to a variety of cellular substrates via ATP-dependent reaction, where the first step involves C-terminal adenylation of glycine residues of SUMO-1 and second step involves thioester bond formation between C-terminal glycine of SUMO-1 and a cysteine residue in SAE2.

Leistungsmerkmale und Vorteile

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Physikalische Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable