SAB3701388

Anti-Human IgG IgA IgM (H+L), F(ab′)₂ fragment, highly cross adsorbed-Biotin antibody produced in goat

affinity isolated antibody, lyophilized powder

• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: goat
• Konjugat: biotin conjugate
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: secondary antibodies
• Klon: polyclonal
• Form: lyophilized powder
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150 kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Immunoglobulin M (IgM) exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190 kDa. The pentameric IgM consists of ten μ-heavy chains, ten light chains and a single J-chain which is linked by disulphide bonds. The μ-chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4. Immunoglobulin A (IgA) is found as two subclasses, that is, IgA1 and IgA2. IgA1 has an extended hinge because of the insertion of a duplicated stretch of amino acids, whereas IgA2 lacks this. IgA exists as a monomer or dimer in the secreted form. Dimeric IgA has a molecular weight of 390 kDa. This antibody can form multimers and its polymerization is essential for transport across epithelia. IgA is crucial as the first line of defence.

Spezifität

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Biotin and Anti-Goat Serum. No reaction was observed against Mouse Serum proteins. This product is suitable for the detection of all Human immuno-globulin classes, isotypes and chain combinations.

Immunogen

Human IgG, IgA and IgM whole molecules

Physikalische Eigenschaften

Antibody format: IgG F(ab′)2

Physikalische Form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Rekonstituierung

Reconstitute with 1.0 mL deionized water (or equivalent).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS06,GHS09
• Signalwort: Danger
• H-Sätze: H302,H311,H411
• P-Sätze: P280 - P301 + P312 + P330 - P302 + P352 + P312
• Gefahreneinstufungen: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2
• Zusätzliche Gefahrenhinweise: EUH032
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable