S2069

Anti-SNAP-29 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-Synaptosomal-Associated Protein 29
• MDL-Nummer: MFCD06798540
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 29 kDa
• Speziesreaktivität: mouse, rat
• Methode(n): indirect immunofluorescence: 1:100 using mouse fibroblast NIH3T3 cell line.; microarray: suitable; western blot: 1:1,000 using whole cell extract of mouse fibroblasts NIH3T3
• UniProt-Hinterlegungsnummer: Q9ERB0
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Snap29(67474) ; rat ... Snap29(116500)

Beschreibung

Allgemeine Beschreibung

Synaptosomal-associated protein 29 kDa (SNAP-29), is a non-neuronal isoform of synaptosomal-associated protein 25 (SNAP-25), originally isolated from a human cDNA library in a yeast two-hybrid screen for proteins interacting with syntaxin 3. It is localized predominantly in intracellular membrane structures specifically with the Golgi apparatus. SNAP-29 is ubiquitously expressed, in neuronal and non-neuronal cells. It codes for a protein which belongs to the N-ethylmale-imide-sensitive factor-attachment protein receptor (SNARE) family of proteins. SNAP-29 gene is mapped to human chromosome 22q11.2.

Spezifität

This sequence is identical in rat SNAP-29 and highly conserved (single amino acid substitution) in human SNAP-29.

Immunogen

synthetic peptide corresponding to the C-terminus of mouse SNAP-29 (amino acids 238-257), conjugated to KLH.

Anwendung

Anti-SNAP-29 antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem./physiol. Wirkung

Synaptosomal-associated protein 29 kDa (SNAP-29) plays an important role in intra-Golgi traffic and regulates a variety of intracellular membrane trafficking pathways. SNAP-29 is capable of participating in various intracellular transport steps, by interacting with different syntaxins and vesicle-associated membrane proteins (VAMPs), specifically localized to different membrane compartments. It is present in synapses and is capable of interacting with syntaxin 1A. For binding to the N-ethylmale-imide-sensitive factor-attachment protein receptor (SNARE) complex, SNAP-29 competes with α-SNAP and subsequently controls synaptic transmission by preventing disassembly of the SNARE complex. It plays a role in the endocytosis of insulin-like growth factor 1 (IGF-1) receptors, by directly interacting with insulin like growth factor1 receptor (IGF1R). Bi-allelic mutations in SNAP-29 is associated with cerebral dysgenesis, neuropathy, ichthyosis, and keratoderma (CEDNIK) syndrome. In infants, deletion of chromosome 22q11.2 results in DiGeorge and Velocardial facial syndrome (22q11.2DS).

Zielbeschreibung

SNAP-29 , a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.