Direkt zum Inhalt
Merck

RAB0010

Sigma-Aldrich

Angiotensin II EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonym(e):

Angiotensinogen, Serpin A8

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352204
NACRES:
NA.32

Speziesreaktivität

mouse, human, rat

Verpackung

kit of 96 wells (12 strips x 8 wells)

Methode(n)

ELISA: suitable
enzyme immunoassay: suitable

Aufnahme

sample type cell lysate
sample type culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 2.62 pg/mL
standard curve range: 0.1-1000 ng/mL

Nachweisverfahren

colorimetric

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... AGT(183)

Allgemeine Beschreibung

The Angiotensin II Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Angiotensin II peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANGII-1 can theoretically detect all active angiotensins, including ANGI, ANGII, ANGIII and ANGIV. However, it does not detect inactive angiotensinogen.

Immunogen

Angiotensin II, synthetic peptide

Anwendung

Angiotensin II EIA Kit has been used for quantitative estimation of serum angiotensin-II.
For research use only. Not for use in diagnostic procedures.
Angiotensin II EIA Kit has been used for analyzing circulating and serum levels of angiotensin II (Ang II) in mice and rats respectively.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem./physiol. Wirkung

Study on in vitro model shows that mechanical stretch induced Ang II release plays a vital role in mediating stretch-induced hypertrophic response. Ang II is implicated in maintaining blood pressure and has an important role in hypertension and in the pathophysiology of cardiovascular disorders. Ang II also has an essential role in growth induction, cell migration and mitosis of vascular smooth muscle cells. It enhances the production of collagen type I and III in fibroblasts, that results in thickening of the vascular wall and myocardium, and fibrosis. Variation in Ang II gene increases the risk of myocardial infarction, hypertension and left ventricular hypertrophy. Ang II expression is associated with heart failure, cardiac remodeling, diabetes, and the proliferative and inflammatory responses to arterial injury. The pharmacological agents such as angiotensin II-converting enzyme inhibitors or angiotensin II receptor antagonists can be used for treating Ang II-induced hypertension and congestive heart failure.

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • RABHRP3HRP-StreptavidinSDB

Piktogramme

Corrosion

Signalwort

Warning

H-Sätze

P-Sätze

Gefahreneinstufungen

Met. Corr. 1

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

EU REACH Annex XVII (Restriction List)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Role of angiotensin II in blood pressure regulation and in the pathophysiology of cardiovascular disorders.
Fyhrquist F
Journal of Human Hypertension, 9 suppl 5, S19-S24 (1995)
Genetic abrogation of adenosine A3 receptor prevents uninephrectomy and high salt-induced hypertension
Yang T, et al.
Journal of the American Heart Association, 5(7), e003868-e003868 (2016)
Autocrine release of angiotensin II mediates stretch-induced hypertrophy of cardiac myocytes in vitro.
Sadoshima J
Cell, 75(5), 977-984 (1993)
Daniela Volonte et al.
The Journal of biological chemistry, 296, 100242-100242 (2021-01-01)
Oncogenic K-Ras (K-RasG12V) promotes senescence in normal cells but fuels transformation of cancer cells after the senescence barrier is bypassed. The mechanisms regulating this pleiotropic function of K-Ras remain to be fully established and bear high pathological significance. We find
Genetic Abrogation of Adenosine A3 Receptor Prevents Uninephrectomy and High Salt-Induced Hypertension.
Yang T
Journal of the American Heart Association, 5(7) (2016)

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.