P0498

Anti-PRL-3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-PTP4A3, Anti-Phosphatase of Regenerating Liver
• MDL-Nummer: MFCD07370459
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~25 kDa
• Speziesreaktivität: human
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:250-1:500 using protease digested tissue sections from human heart by biotin/ExtrAvidin^®-peroxidase staining; immunoprecipitation (IP): 0.5-1 μL using preparation containing recombinant human PRL-3; western blot (chemiluminescent): 1:500-1:1,000 using whole extract of human HepG2 cells
• UniProt-Hinterlegungsnummer: O75365
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PTP4A3(11156) ; mouse ... Ptp4a3(19245)

Beschreibung

Allgemeine Beschreibung

Phosphatase of regenerating liver (PRL-3), also known as protein tyrosine phosphatase type IVA, member 3 (PTP4A3) is a dual-specificity phosphatase. It belongs to the phosphatases of regenerating liver family. The PTP4A3 gene is localized on human chromosome 8q24.3.

Spezifität

Anti-PRL-3 recognizes human phosphatase of regenerating live (PRL)-3 and does not cross-react with human PRL-1 and PRL-2.

Immunogen

Synthetic peptide corresponding to amino acid residues of human PRL-3 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.

Anwendung

Anti-PRL-3 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem./physiol. Wirkung

Phosphatase of regenerating liver (PRL-3) promotes epithelial-mesenchymal transition. It also plays an essential role in cancer cell migration, invasion and metastasis. It dephosphorylates serine, threonine and tyrosine residues. The protein is overexpressed in various cancers. PTP4A3 associates with transcription factors and regulates gene transcription. In addition, it also regulates breast cancer cell proliferation and invasion. Thus, this protein acts as a potent therapeutic target for breast cancer.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Rechtliche Hinweise

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)