OGS561

PSF-OXB18 - VERY STRONG E.COLI PROMOTER PLASMID

plasmid vector for molecular cloning

• Synonym(e): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• UNSPSC-Code: 12352200
• NACRES: NA.85

Eigenschaften

• Form: buffered aqueous solution
• Mol-Gew.: size 3858 bp
• Bakterienauswahl: kanamycin
• Replikationsursprung: pUC (500 copies)
• Peptidspaltung: no cleavage
• Promoter: Promoter name: OXB18; Promoter activity: constitutive; Promoter type: bacterial
• Reportergen: none
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

PSF-OXB18 - VERY STRONG E.COLI PROMOTER PLASMID that allows the production of protein in bacteria. The plasmid contains our standard multiple cloning site downstream of the OXB18 promoter. We developed this promoter in house by modifying the RecA E. coli promoter so that it no longer repressed by LexA and also incorporates a series of mutations that make it slightly weaker. This is part of an extended product range of bacterial promoters with different transcriptional activities. OXB20 is the strongest in this range and OXB1 is the weakest. This plasmid vector does not require an inducing agent for activity.

Promoter Expression Level: This molecular cloning vector contains a very strong constitutive E. coli promoter that was derived from the RecA promoter by random mutagenesis. It is part of our constitutive bacterial promoter range. This promoter (OXB18) shows the very high levels of expression in the range with OXB1 showing the lowest level and OXB20 showing the highest level of expression. This vector require no inducing agent for expression.

Anwendung

Cloning in a gene: PSF-OXB18 - VERY STRONG E.COLI PROMOTER PLASMID has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.

Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.

The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.

Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.

Sequenz

To view sequence information for this product, please visit the product page

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable