OGS3161
PSF-OXB20-NH2-OMPT - OMPT SECRETION PLASMID
plasmid vector for molecular cloning
Synonym(e):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
UNSPSC-Code:
12352200
Rekombinant
expressed in E. coli
Form
buffered aqueous solution
Mol-Gew.
size 3908 bp
Bakterienauswahl
kanamycin
Replikationsursprung
pUC (500 copies)
Peptidspaltung
no cleavage
Lage der Peptid-Tags
N-terminal
Promoter
Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial
Reportergen
none
Sekretionssignal
OmpT
Versandbedingung
ambient
Lagertemp.
−20°C
Allgemeine Beschreibung
This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.
About the Peptide Tag:This plasmid contains an Outer Membrane Protein T (OmpT) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. .
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
About the Peptide Tag:This plasmid contains an Outer Membrane Protein T (OmpT) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. .
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
Sequenz
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Hinweis zur Analyse
To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.
Sonstige Hinweise
Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.
Rechtliche Hinweise
Oxford Genetics is a trademark of Oxford Genetics Ltd
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Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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